Withdrawal statementThe authors have withdrawn this manuscript because refined experiments with a remodeled two-color MINFLUX setup featuring improved accuracy and precision of the two-color registration, do not support the reported Chasse-Inchworm stepping mechanism of kinesin-1.
The Chasse-Inchworm mechanism was postulated based on recurrent observations of separations of 0 and 16 nanometers between a green-absorbing and a red-absorbing fluorophore, each respectively attached to the two motor domains of kinesin-1. However, the same experiments performed with the improved two-color MINFLUX setup revealed merely separations of 0 and 8 nanometers, aligning with the widely accepted hand-over-hand mechanism.
Scheiderer, L. et al. · CC-BY 4.0